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plentiv2 vector  (Addgene inc)


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    Addgene inc plentiv2 vector
    Plentiv2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plentiv2+vector/lentiCRISPR+v2+(Plasmid+%2352961)/us12480163-1942-36-38
    Average 96 stars, based on 6150 article reviews
    plentiv2 vector - by Bioz Stars, 2026-10
    96/100 stars

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    Produced:

    Article Title: Genome-scale CRISPR-Cas9 screen identifies druggable dependencies in TP53 wild-type Ewing sarcoma
    Article Snippet: RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO. .. Lentivirus was produced by transfecting HEK-293T cells with the pLentiV2 vector (Addgene plasmid 52961) and the packaging plasmids pCMV8.9 and pCMV-VSVG according to the FuGENE 6 (Roche) protocol. .. For lentiviral transduction, Ewing sarcoma cells were incubated with 2 ml of virus and 8 μg/ml of polybrene (Sigma-Aldrich).

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO. c. Lentivirus Production and Transduction Lentivirus was produced by transfecting HEK-293T cells with the pLentiV2 vector (Addgene plasmid 52961) and the packaging plasmids pCMV8.9 and pCMV-VSVG according to the FuGENER 6 (Roche) protocol. .. For lentiviral transduction, Ewing sarcoma cells were incubated with 2 ml of virus and 8 μg/ml of polybrene (Sigma-Aldrich).

    Plasmid Preparation:

    Article Title: Genome-scale CRISPR-Cas9 screen identifies druggable dependencies in TP53 wild-type Ewing sarcoma
    Article Snippet: RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO. .. Lentivirus was produced by transfecting HEK-293T cells with the pLentiV2 vector (Addgene plasmid 52961) and the packaging plasmids pCMV8.9 and pCMV-VSVG according to the FuGENE 6 (Roche) protocol. .. For lentiviral transduction, Ewing sarcoma cells were incubated with 2 ml of virus and 8 μg/ml of polybrene (Sigma-Aldrich).

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO. c. Lentivirus Production and Transduction Lentivirus was produced by transfecting HEK-293T cells with the pLentiV2 vector (Addgene plasmid 52961) and the packaging plasmids pCMV8.9 and pCMV-VSVG according to the FuGENER 6 (Roche) protocol. .. For lentiviral transduction, Ewing sarcoma cells were incubated with 2 ml of virus and 8 μg/ml of polybrene (Sigma-Aldrich).

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    Transduction:

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. RG7388 (ApexBio Technology), GSK2830371 (Selleck Chemicals), P5091 (Sigma-Aldrich), doxorubicin (Cell Signaling), etoposide (Selleck Chemicals), and vincristine (Selleck Chemicals) were solubilized in DMSO. c. Lentivirus Production and Transduction Lentivirus was produced by transfecting HEK-293T cells with the pLentiV2 vector (Addgene plasmid 52961) and the packaging plasmids pCMV8.9 and pCMV-VSVG according to the FuGENER 6 (Roche) protocol. .. For lentiviral transduction, Ewing sarcoma cells were incubated with 2 ml of virus and 8 μg/ml of polybrene (Sigma-Aldrich).

    Generated:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    shRNA:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    Control:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.



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